(C) 2012 Elsevier Ltd. All rights reserved.”
“The increasing use of the baculovirus expression vector system (BEVS) has generated significant interest into techniques for quantifying baculovirus stocks. One method involves the use of quantitative real-time polymerase chain reaction (PCR). This study investigated simplifying baculovirus sample preparation for quantitative Real Time PCR to provide an alternative
to current kit-based preparation methods. To achieve this goal, combinations of freeze/thaw cycles and Triton X-100 treatment were investigated. A treatment with only Triton X-100 was found to be sufficient to provide a simple, rapid and cheap alternative to kit-based preparation methods. This study also examined other factors such
as primer choice SN-38 chemical structure to further examine the process of baculovirus quantitation by qPCR. (C) 2012 Elsevier B.V. All rights reserved.”
“We have developed an optimized procedure using dual size exclusion/affinity hydrogel nanoparticles to capture and comparatively analyze low molecular mass proteins directly from biological samples. The method described facilitates charge- and size-dependent protein binding, direct analysis by MS or other means and is highly reproducible. A comparative analysis of the low molecular mass proteome of plasma following freeze-thaw immediately after venipuncture is used to illustrate proof-of-concept. The technique described is rapid and may be easily reproduced in any laboratory.”
“Partial lesion (50%) of the nigro-striatal dopaminergic www.selleckchem.com/products/a-1155463.html pathway induces compensatory increase in dopamine release from the remaining neurons and increased extracellular oxidative stress (OS-ec) in the striatum. The present study
was designed to explore the role of monoamine oxidase types A and B (MAO-A, MAO-B) in producing this increased oxidative stress. Lesion of the dopaminergic pathways in the CNS was produced in rats by intra-cerebroventricular injection of 6-hydroxydopamine (6-OHDA; 250 mu g) and striatal microdialysis was carried out 5 weeks later. Striatal OSec was determined by measurement of oxidized derivatives of the marker molecule N-linoleyl-tyrosine. Striatal tissue MAO-A science activity was unchanged by 6-OHDA lesion but MAO-B activity was increased by 16%, together with a 45% increase in glial cell content. The selective MAO-B inhibitor rasagiline (0.05 mg/kg s.c. daily for 14 days) did not affect microdialysate dopamine concentration [DA(ec)] in sham-operated rats, but decreased OSec by 30%. In lesioned rats, rasagiline decreased [DA(ec)] by 42% with a 49% reduction in OSec. The decrease in [DA(ec)] was reversed by the dopamine D2 receptor antagonist sulpiride (10 mg/kg s.c.). The selective MAO-A inhibitor clorgyline (0.2 mg/kg s.c. daily for 14 days) increased striatal [DA(ec)] by 72% in sham-operated rats with no change in OSec. In lesioned rats clorgyline increased [DA(ec)] by 66% and decreased OSec by 44%.