37 0.45 0.58 PSPPH_2918
membrane protein, putative 0.37 0.13 0.12 PSPPH_2919 carbonic anhydrase, putative 0.27 0.18 0.19 osmC hydroperoxide resistance protein OsmC 0.22 0.45 0.63 PSPPH_4984 prophage PSPPH06, site-specific recombinase, phage integrase family 0.11 0.25 0.62 PSPPH_2219 transcriptional regulator, AsnC family 0.09 0.15 0.59 PSPPH_3916 membrane protein, putative 0.07 0.01 0.02 PSPPH_2216 zinc carboxypeptidase domain protein 0.04 0.20 0.54 PSPPH_2747 transcriptional regulator, Cro/CI family 0.49 0.59 PSPPH_B0005 transcriptional regulator, Cro/CI family 0.46 0.45 learn more PSPPH_3928 ABC transporter, binding protein 0.34 0.63 PSPPH_0189 ATP-dependent DNA helicase RecG 0.34 0.42 PSPPH_4962 prophage PSPPH06, C4-type zinc finger protein, DksA/TraR family 0.24 0.16 PSPPH_0194 ActC family protein 0.24 0.56 PSPPH_2746 dipeptide ABC transporter, ATP binding protein 0.14 0.33 PSPPH_0970 O-methyltransferase I 0.12 0.24 PSPPH_0592 high-affinity branched-chain amino acid ABC transporter, permease protein BraE 0.08 0.30 eda2 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase 0.43 PSPPH_4761 glutathione S-transferase family protein 0.43 PSPPH_1737 transcriptional regulator, LysR family 0.42 PSPPH_4723 molybdate transport regulator ModE, putative 0.41 PSPPH_3100 isocitrate dehydrogenase, NADP-dependent 0.40 PSPPH_3284 beta-lactamase 0.34 PSPPH_1244 transcriptional regulator,
AsnC family 0.30 PSPPH_3265 Entinostat supplier acetyltransferase, GNAT family 0.27 pilo type IV pilus else biogenesis protein PilO 0.16 PSPPH_5152 pyridoxal kinase 0.43 The table includes genes that shown ≤ 0.5 check details fold change in expression level. L Bean leaf extract, A apoplastic fluid and P Bean pod extract. ORF nomenclature corresponding to 1448A reference sequenced strain. For a complete list of all statistically repressed genes please consult Additional File 1. Figure 1 Effects of plant extracts on cultures grown in M9 minimal media. Growth of P. syringae pv. phaseolicola NPS3121 in M9 minimal medium supplemented with bean leaf extract, apoplastic fluid and bean pod extract. At mid log phase (OD600 nm 0.6) the cultures were supplemented with 2% of plant
extracts. Culture density was measured by spectrophotometry after induction during 6 hours. The bean extracts increased bacterial growth rate on supplemented media in comparison to non supplemented media. Figure 2 Overview of the microarray strategy. A library of chromosomal DNA fragments of P. syringae pv. phaseolicola NPS3121 (Psp NPS3121) was constructed in the pUC19 vector and introduced into the E. coli Top10 strain. 30% (2880 clones) of the genomic library was sequenced, aligned and annotated against the complete genome of P. syringae pv. phaseolicola 1448A. This strategy allowed selection of 1911 clones that provided approximately 1× coverage of the genome. The fragments of 1911 clones were amplified by PCR reaction, and the products were printed on a microarray slide.