The therapeutic potential of induction or suppression of autophag

The therapeutic potential of induction or suppression of autophagy in cancer treatment undoubtably depends on understanding the role of autophagy in cancer cells. Paclitaxel (Taxol) is an effective mitotic inhibitor and apoptosis inducer. It has been widely used in chemotherapy for lung cancer, breast cancer, ovarian cancer, and Kaposi’s sarcoma

[6]. It has been shown that in non-small cell lung carcinoma cells, while paclitaxel treatment leads to apoptosis, paclitaxel also induces an autophagic response that plays a Z-IETD-FMK price protective role impeding the eventual cell death [7]. While some recent studies demonstrated that paclitaxel treatment led to increased autophagy in lung cancer cells and osteosarcoma cells, and inhibition of autophagy increased the Selleckchem CP 690550 cytotoxic sensitivity of cells to paclitaxel [7, 8], Veldhoen

et al. reported that paclitaxel could inhibit autophagy in breast cancer cells by blocking activation of the class III phosphatidyl inositol 3 kinase, Vps34, and autophagy sensitized cells to paclitaxel toxicity [9]. These conflicting results suggested that the treatment effects of paclitaxel on autophagy might be cell-type dependent. Recently, it has been demonstrated that paclitaxel exhibits check details preferential toxicity to folliculin (FLCN)-deficient renal cell carcinoma (RCC) line, UOK257, a cell line which originated from a patient with Birt–Hogg–Dube (BHD) syndrome [10]. BHD syndrome, caused by FLCN mutations, is an autosomal dominant genetic disease characterized by susceptibility to renal cancer, 5-FU molecular weight renal and pulmonary cysts, and noncancerous tumors of the hair follicles [11]. Function of FLCN has been linked to mTOR and AMPK signaling pathways [12, 13]. In addition, FLCN was reported to be involved in apoptosis [12,

14–16]. Furthermore, FLCN was recently found to be associated with the activity of LC3-mediated autophagic program [17]. These findings might provide new insights into the treatment of BHD disease. While early-stage bilateral renal cancer associated with BHD disease could be managed with partial nephrectomy, an effective cure for BHD disease associated renal cancer has not been established. The preferential toxicity of paclitaxel to UOK257 FLCN-deficient cell line suggested that paclitaxel might be a candidate anticancer drug for FLCN-deficient tumors [10]. To further determine the cellular response of FLCN-deficient cell lines treated with paclitaxel, here we examined apoptosis and autophagy induced by paclitaxel in human renal cancer cell lines with or without FLCN expression. Our results indicated that autophagy induced by paclitaxel in FLCN-null renal cancer cells plays a protective role, and the inhibition of autophagy could increase apoptosis induced by paclitaxel treatment in these cancer cells.

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