The solidified plates were bored with 5 mm dia cork bored. The plates with wells were used for

the antibacterial studies. Antibacterial activity of oleananoic acid acetate was done by well diffusion method.9 The prepared culture plates were ABT-199 cost inoculated with selected strains of bacteria using streak plate method. Wells are made on the agar surface with 6 mm cork borer. The compound was poured into the well using sterile syringe. The plates were incubated at 37 °C ± 2 °C for 24 h. The concentration of the compound was 25 μg/mL. The plates were observed for the zone formation around the wells was measured in mm (millimeter). For each treatment three replicates were maintained. The diameter of inhibition zones was measured in mm and the result were recorded inhibition zones with diameter less than 12 mm were considered ad having no antibacterial activity. Diameters between 12 and 16 mm were considered moderately active Fulvestrant purchase and these with ≥16 mm were considered highly active.9 The structure of Oleananoic acid acetate as shown in Fig. 1. The results of the antibacterial activity data were tabulated at Table 1. Oleananoic acid acetate was obtained white solid which gave positive Lieberman–Burchard test for triterpenoids.8 IR spectra showed absorption frequency at 3384,

this indicates the presence of (O H) stretch for hydroxyl group, which was bonded with (C O) of an acid obtained the signal at 1589. This two supports the carboxylic acid ( COOH), functional group at position of C-28. The frequency at 2923 is due to (C H) stretch for an alkane and absorption showed at 1499, 1299 is due to presence of ( CH3, CH2) group in the molecule. The absorption frequency at 1021 signifies

cycloalkane. The assigned NMR spectra were in good agreement with literature value. IN 1H NMR spectra, the chemical shift obtained at 4.161 is indicated the (H-3) bonded with oxygen group. The signal at 0.809, 1.255 and 1.74 is due to presence of ‘CH’ group Ketanserin and signal at 1.85 due to CH2 group. The 1HNMR showed shift at 0.830, 0.688, 0.994, 0.905 attribute the CH3 groups. The presence of Olean skeleton was confirmed in the 13C NMR spectrum with the signals in the region δ 11.46–38.31 ppm at 26 and at 23.77 attributed to seven methyl groups and absence of double bond at the position of C-12, C-13. 13C NMR shows shift at 180.3 corresponds to ( COOH) bond at the position of C-28 and 167, 10.60 corresponds to (C O) linkage at position C-11, C-21. In EI-MS, the molecular ion not observed but the molecular ion (M+ + H) of compound was observed at m/z-501 (10) in the ESI-MS respectively showing its molecular formula C32H52O4 and fragmented peaks at for EI-MS – 459 (5), 485 (5) and for ESI-MS – 457 (7), 485 (58). IR absorption band at 2923 is due to C H stretch for an alkane. This account for the high degree of saturation of the molecule. This also supported by 13C NMR, the signal obtained at 36.6 & 23.77.