The persistence seen with the subject-specific LAB strains cultivated from faeces is also interesting in this regard. Commercialisation of LAB strains for probiotic use is dependent on a number of factors, however, from our study and other work, it appears that many commercialised LAB strains are genotypically identical to reference strains deposited in recognised culture collections (Table 2). The
fingerprinting strategy described herein could be used to select LAB Selleck Talazoparib strains with better persistence in human populations by screening a large population of healthy people, and selecting the dominant LAB strain types for evaluation as probiotics. Conclusion We have shown that specific Lactobacillus strains consumed as part of a feeding study can be tracked through gastrointestinal passage via a colony-based strain typing strategy. The ability to identify specific LAB strains in faeces after human consumption provides a means to answer many important questions concerning the clinical use of probiotics. Our fingerprinting strategy could be used to identify the presence of the LAB isolates of the same genotype as potential probiotics prior to their administration in clinical trials, therefore allowing outcome measures dependent on the
probiotic to be distinguished from those dependent on individuals which may naturally carry the same LAB strain. Overall, the successful application {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| of molecular epidemiological techniques to cultivable bacterial populations within the human gut provides a platform for future systematic studies on the development of probiotics, as well as a rapid means to assess the strain diversity in healthy versus diseased
humans. Methods Bacterial strains and cultivation Lactobacillus reference strains were obtained from the Belgium Coordinated Collections of Microorganisms (BCCM; http://bccm.belspo.be/). Additional commercial LAB isolates were obtained from Cultech Ltd (Port Talbot, Wales, UK) or cultured directly from commercially marketed probiotic products as described below; a list of the strains used in this study is shown in Table 2. All strains of LAB were cultivated on MRS agar or in MRS broth (Oxoid, Basingstoke, UK) for 24 to 72 hours at 37°C. Commercial probiotic capsules and powders were resuspended in 5 ml MRS broth Methane monooxygenase and serial dilutions plated onto MRS agar. To improve the isolation of LAB species from faecal samples, the semi-selective capacity of MRS agar was enhanced by the additional of 120 units per ml of Polymixin B (MRS-P medium; Polymixin B from, Sigma-Aldrich, Gillingham, UK). Fresh Selleck FG 4592 growth of purified faecal isolates was swabbed and resuspended in MRS broth containing 8% vol/vol dimethylsulphoxide prior to storage at -80°C. Frozen strains were revived by swabbing the surface of the frozen resuspension and plating onto MRS agar followed by incubation as above.