The labeling efficiency of Ga-68-NOTA-MSA was greater than 99% at room temperature, and its stability
was greater than 99% at 4 h. Biodistribution and micro-PET studies of Ga-68-NOTA-MSA showed high liver and spleen uptakes after intravenous injection. Ga-68-NOTA-MSA injected into a footpad rapidly migrated to the lymph node.
Conclusions: Ga-68-NOTA-MSA was successfully developed as JQ1 a novel SLN imaging agent for PET. NOTA-MSA is easily labeled at high efficiency,,and subcutaneously administered Ga-68-NOTA-MSA was found to migrate rapidly to the lymph node. (C) 2011 Elsevier Inc. All rights reserved.”
“We investigate a definition of biological information that connects population genetics with the tools of information theory by focusing on
the distribution of genotypes found in a population. Previous research has treated loci as non-interacting by making specific approximations in the calculation of information-theoretic quantities. We expand earlier mathematical forms to include epistasis, or interactions between mutations at all pairs of loci. Application of our improved measure of biological information to evolution on two-locus, two-allele fitness landscapes demonstrates that mutual information between loci reflects epistatic interaction of mutations. Finally, we consider four-locus, two-allele fitness landscapes with modular structure. As modular interactions are inherently epistatic, we demonstrate that our refined approximation provides insight into the underlying structure of these non-trivial fitness landscapes. (c) 2010 Elsevier Ltd. All rights reserved.”
“Purpose: Two variants of Annexin A5 (Cys2-AnxA5 and Cys 165-AnxA5) selleck inhibitor were labelled with Gallium-68 in order to evaluate their biological properties.
Procedures: Biodistribution and pharmacokinetics
of the radiotracers were studied with mu PET in healthy mice and in a mouse model of hepatic apoptosis. mu PET imaging after IV injection of the tracers in combination with mu MRI was performed in Daudi tumor bearing mice before and after treatment with a combination of chemotherapy and radiotherapy.
Results: The biodistribution data indicated a fast urinary clearance with only minor hepatobilliary clearance, although a high retention in the kidneys was observed. Animals treated with anti-Fas most showed a 3 to 8 times higher liver uptake as compared to healthy animals. Tumor uptake of Ga-68-Cys2-AnxA5 and Ga-68-Cys165-AnxA5 was low but significantly increased after therapy.
Conclusion: Both Ga-68-Cys2-AnxA5 and Ga-65-Cys165-AnxA5 show a clear binding to apoptotic cells and are promising tracers for rapid evaluation of cancer therapy. (C) 2011 Elsevier Inc. All rights reserved.”
“I developed a genetic-physiological model to investigate the molecular mechanisms regulating annual and perennial life histories, and theoretically explored local adaptation caused by environment-specific selection.