Preparation of the reference standard solution The standard solutions were prepared by adding a properly weighted total of anthranilic acid, benzoic acid, syringic acid, salicylic acid to your volumetric flask and dissolved with 10 mL MeOH to really make the ultimate focus deubiquitinating enzyme inhibitor of 12 38 mg/mL respectively. The concentrations of tryptanthrin, indirubin and indigo were 0. 4 10 mg/mL this season dimethyl-sulfoxide methanol solution. 2. 5 Preparation of sample solution R. isatidis was smashed in to a powder of 24 mesh, and 3 g of the powder was extracted and precisely weighed with 30mL of EtOAc in a ultra-sonic water bath for 1 h. After extraction, the solution was filtered by a triangular glass funnel under negative pressure. The filtrate was concentrated to dryness in a rotary evaporator by evaporation and vacuum. The residue was redissolved with 3 mL MeOH twice, and then filtered through a 0. 45-mm abs micropore film to generate the sample Plastid solution. The 20 mL solution was injected in to liquid chromatography system for research. Each sample was prepared with the above method for LC analysis. 2. 6 Analysis of LC fingerprints 2. 6. 1 Selection of variable wavelength To choose the appropriate wavelengths, sample no. 8 solution was selected randomly to look wavelengths so that you can improve the response signal of compounds in samples up to possible and to secure a sufficiently large number of detectable peaks on the LC chromatogram. DAD full check was employed here. After determining the recognition wavelengths, 20 mL sample solutions from 11 origin R. isatidis were injected into the LC system respectively, and the chromatograms were run for 150 min to ensure that just about all arrangements of the samples could be eluted out. 2. 6. 3 Fingerprint installation coupled with the multiwavelength combination Anacetrapib msds technique The multi wavelength combination technique could better reflect just about all chemical compositions and their contents in the complex samples, especially TCMs that draw particular focus on play a standard role in disease prevention and treatment through multi element and multi goal device. Inside the actual process, use of short wavelength and the gradient elution method was often a very popular short-cut, but this approach also introduced imbalanced baseline chromatogram, neglected weak-signal mountains, lacked longwavelength signs and so on. On the basis of the peak signal power of sample compositions underneath the detection, a complete retention time was reasonably split into a number of retention time segments, and then several peak signal strong chromatogram segments corresponding to their respective retention time segments were recombined together. The schematic diagram of LC fingerprint utilizing the multiple wavelength mix technique is shown in Fig. 1.