Neurons were treated with 100 nM of the

panTrk inhibitor K252a for 24 hr or 100 ng/ml BDNF for 30 min. Coverslips were then fixed with 4% PFA for 20 min, washed with PBS, incubated with 1 M NH4Cl for 15 min, washed, and then mounted with Mowiol. A construct carrying a tandem mCherry-EGFP was used as positive control for intramolecular FRET. Two constructs carrying mCherry and EGFP (Clontech) separately were cotransfected to provide a negative control. FRET/FLIM measurements were performed as in Zhang et al. (2013). For details see the Supplemental Experimental Procedures. SCH727965 manufacturer See the Supplemental Experimental Procedures. See the Supplemental Experimental Procedures. Comparisons between two groups were performed using one-sample or two-sample two-tailed Student’s t test. One-way or two-way ANOVA followed by post hoc Student’s t test with Holm’s or Bonferroni correction were used for multiple comparisons. Distributions were analyzed using Pearson’s χ2 test. Comparisons between cumulative probability

plots were performed using two-sample Kolgomorov-Smirnov (K-S) test. Significance was accepted to p < 0.05. Bars represent SEM. We thank Ilaria Napoli and Tiziana Girardi for preliminary data. We are grateful to Evita Mohr and Joachim Kremerskothen for the PABP1 and SYNCRIP antibodies. We are grateful to Elien Theuns, Jonathan Royaert, Karin Jonkers, Ingeborg Beheydt, and Roel van der Schors for technical help and to Bing Yan for viral production. We are thankful to Paul Ribociclib Woolley, Carolina Barillas, and Giovanni Isotretinoin Chillemi for comments on the manuscript and to Sebastian Munck, coordinator of LiMoNe, for his advice. S.D.R. was supported by the Associazione Italiana Sindrome X Fragile and by a Fonds Wetenschappelijk Onderzoek (FWO) grant to C.B. (FWO G.0705.11); E.P. was supported by an FWO (aspirant fellowship); D.D.M was supported by an FWO grant to C.B. (FWO G.0705.11); E.F. was supported by an Intra-European

Marie Curie Fellowship FP7. We are indebted to the Schizophrenia subgroup of the Psychiatric Genetics Consortium for providing access to the results of their meta-analysis. This work was supported by grants from the following agencies: Queen Elisabeth Foundation (Belgium), CARIPLO, FWO (FWO G.0705.11), VIB, and Telethon (GGP10150) to C.B.; HEALTH-2009-2.1.2-1 EU-FP7 “SynSys” to A.B.S., S.G.N.G., and C.B.; FP7 GENCODYS and EU-FP7 “EUROSPIN” to A.B.S. and S.G.N.G.; Wellcome Trust to S.G.N.G.; and the Center for Medical Systems Biology (CMSB) to A.B.S. Nikon microscope used in this study was acquired through a Hercules Type 1 AKUL/09/037 to Wim Annaert. We are very grateful to Eef Lemmens for administrative support. “
“Homeostatic signaling systems are believed to interface with the mechanisms of learning-related plasticity to achieve stable, yet flexible, neural function and animal behavior.