lectularis for O. horni (RA) and Nasonia vitripennis for C. heimi

(TLR). Three O. horni (T1, TER30 and T21) and two Odontotermes spp. (T3 and THYD) formed two separate sister clades with Wolbachia from K. flavicollis (Fig. 2). Odontotermes horni (MCT) and C. heimi (TERMITE3) were found to be divergent within representatives of F supergroup Wolbachia included in this analysis (Fig. 2). All the strains clustering in F and B supergroups on the basis of MLST also grouped with the respective supergroup Wolbachia on the basis of 16S rRNA gene sequences. Odontotermes Wolbachia were found close to Microcerotermes sp. (RA), Mansonella (MCT and G29), whereas four Wolbachia from Odontotermes spp. (THYD, T1, TER30 and T21) formed a separate sister clade divergent from the Coptotermes clade within supergroup F. O. horni (T2) clustered Hedgehog antagonist with supergroup B Wolbachia included

in the analysis (Fig. 3). The phylogenetic tree structure revealed two major clusters for Odontotermes spp. from this study (Fig. 4). Morphologically well-identified 17-AAG seven O. horni showed strong clustering with O. horni (EU258629 and EU258630) from the GenBank database reported from Punjab, India. Five other Odontotermes species identified morphologically up to the genus level only formed a sister clade with Odontotermes zambesiensis and O. horni (Fig. 4). Morphologically well-identified two Coptotermes hemi were phylogenetically close to the reported Indian C. heimi (AY558908) from the GenBank database (Fig. 4). This is the first report of the occurrence of Wolbachia in the Odontotermes genus. Infection of Wolbachia in C. heimi has also been detected for the first time, although its occurrence in Coptotermes species (C. acinaciformis and C. secundus) Niclosamide has been reported earlier. During this study, all positive PCR-purified

products were sequenced directly with the same primers used for amplification. The possibility of double or multiple infections in the 14 positive colonies was unlikely as readable chromatograms were obtained, suggesting amplification of a unique copy during the reaction, although this cannot be ruled out. The remarkable diversity of Wolbachia strains in the examined termites was detected with the help of MLST. Supergroup B and F Wolbachia were found in both the genera under study (Odontotermes and Coptotermes) (Table 1). None of the Wolbachia found in this study clustered with those previously found in supergroup H (Zootermopsis spp.) and supergroup A (Cubitermes sp. and I. snyderi). According to Baldo et al. (2006), when the complete set of the five MLST gene sequences cannot be obtained for a strain, single-gene alleles and partial MLST allelic profiles can be submitted to the database. Partial data provide useful allele diversity information, allowing the profile database to grow.