Laboratory Investigation (2012) 92, 178-190; doi: 10.1038/labinvest.2011.162; published online 14 November 2011″
“Recent studies have suggested a protective role of hsp27 against atherosclerosis and transplant graft vasculopathy. Here we have investigated the effects of over-expression of wild-type hsp27 and its phosphorylation mimics on proliferation of human endothelial cells (ECs) and smooth muscle cells (SMCs). ECs and SMCs cultured from human blood vessels or cells lines (human microvascular endothelial cell line and buy Volasertib human telomerase reverse transcriptase subunit SMC) were infected with adenovirus containing
DNA from wild-type hsp27, hyperphosphorylated hsp27 mimic (3D hsp27), hypo-phosphorylated hsp27 mimic (3A hsp27) or anti-sense hsp27, and proliferation measured over the next 5 days. Protein extracts
from infected cells were subjected to proteomic analysis using 2-D DIGE. Over-expression of 3D hsp27 and anti-sense hsp27 but not 3A hsp27 mimic caused significant inhibition of proliferation of ECs and SMCs. Proteomic analysis focussed on proteins that were significantly down-regulated by CH5183284 in vivo the 3D hsp27 mutant. The cell cycling proteins stathmin, cofilin and ubiquitination enzymes fullfilled these criteria. I-D Western blots of infected human microvascular endothelial cell line and human telomerase reverse transcriptase subunit SMC confirmed down-regulation of stathmin, cofilin and ubiquitination enzymes by 3D hsp27. The phosphorylation status of bsp27
is an important regulator of proliferation of human vascular ECs and SMCs; possibly contributing to cardiovascular protection.”
“Three-dimensional fluid-attenuated inversion recovery (3D-FLAIR) imaging 24 h after intratympanic gadolinium injection (IT method) or 4 h after intravenous injection (IV method) has been used to visualize endolymphatic hydrops in M,niSre’s disease. The aims of this study were to evaluate the difference in gadolinium distribution in cochlear perilymph between the two methods by comparing the enhancement of the basal and apical turns and clarify the pharmacokinetics in cochlear perilymph.
A total of 24 ears of 22 patients who underwent Regorafenib the IT method (gadolinium-diethylene-triamine pentaacetic acid was diluted eightfold with saline) and 28 ears of 17 patients who underwent the IV method (double dose of gadoteridol (0.5 mmol/ml); 0.2 mmol/kg body weight in total amount) at 3 T was analyzed retrospectively. Regions of interest of the perilymph of the cochlear basal turn (B), of the apical turn (A), and the medulla oblongata (M) were determined on each patient. The signal intensity ratios between B and M (BMR), A and M (AMR), and A and B (ABR) were subsequently evaluated.
The IT-BMR (2.63 +/- 1.22) was higher than the IV-BMR (1.46 +/- 0.45) (p < 0.001). There was no significant difference between the IT- (1.