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for indoor handling. J Plant Res 2000, 113:507–509.CrossRef 37. Becard G, Fortin JA: Early events of vesicular arbuscular mycorrhiza formation on Ri T-DNA transformed roots. New Phytolog 1988, 108:211–218.CrossRef Competing interests The authors declare that they have no conflict of interest. Authors’ contributions YT and MN generated the strains used. YT and HM performed most of the analyses. YT, HM, KK and MU designed the study and drafted the manuscript. All authors read and approved the S63845 chemical structure final manuscript.”
“Background Syphilis, caused by Treponema pallidum ssp. pallidum (T. pallidum), is a sexually transmitted multistage disease with a diagnosis based on clinical symptoms, serological findings and other methods such as direct detection of treponemes by microscopy. In the 1990s, PCR-based methods for direct detection of AMN-107 treponemal DNA were developed [1]. Since then, several improvements

in these tests have been published which have increased sensitivity and specificity [2–9] as well as the ability to detect the presence of several pathogens simultaneously in the same reaction using multiplex PCR [10, 11]. A major advantage of buy Emricasan PCR–based methods in syphilis diagnostics is the potential for subsequent molecular typing of syphilis treponemes. Although several treponemal genomic loci were tested relative to their suitability for molecular typing [12–14], most molecular typing

studies of treponemal DNA are performed using CDC typing [15]. The method involves detection of the number of 60-bp tandem repeats in the arp (acidic repeat protein) gene and restriction fragment length polymorphism (RFLP) analysis of the 3 PCR-amplified tpr genes (tprE, G, J). FER In 2010, the CDC method was modified by addition of sequencing of TP0548 [14] or by determination of the number of G repeats within the rpsA gene (TP0279) [16]. Recently, a sequencing-based molecular typing scheme based on sequencing of the TP0136, TP0548 and 23S rRNA genes was introduced [17]. Moreover, the sequence variants of TP0136, TP0548 and 23S rRNA genes have been shown to independently combine with variants of the arp and tpr genes [17]. In this communication, we compare CDC typing with sequence-based molecular typing in a group of patients with two or more parallel samples (i.e. taken at the same time) that were PCR-positive for treponemal DNA. Moreover, the variability of gene sequences, length and RFLP genotypes are compared in two types of clinical specimens (i.e. swab and whole blood samples).