Conclusion: There is a need to develop continuing medical education of palliative care and pain management for medical students and physicians of southwest hospitals. Key Word(s): 1. Cancer Pain; 2. China; Presenting Author: LIMENG TING Additional Authors: HUANGJIE AN Corresponding Author: HUANGJIE AN Affiliations:
guangxi medical university Objective: To investigate correlation between Sphingosine kinase 1(Sphk1) and vasculogenic mimicry (VM) formation in colon cancer cells in vitro. Methods: Human colon cancer cell line HT-29 cells were divided into three groups: HT-29 cells were treated with 100 nm/L Phorbol 12-myristate 13-acetate (PMA) as the Sphk1 activation group, 50 μmol/L N, N-dimethyl-D-erythro-sphingosine (DMS) as suppression group, and the equal volume of culture medium as control group. After treatment, cell www.selleckchem.com/products/Nolvadex.html proliferation was detected by MTT, cell invasiveness and migration were assessed by Transwell chamber assays. The effect of apoptosis was observed by transmitting electronic microscope (TEM). The VM formation was observed by the three dimensional culture. The mRNA and protein expression
of VEGF was evaluated by QT-PCR and Western blot. The secretion of VEGF was detected by ELISA. Results: After treated with DMS, cell click here proliferation, invasion and migration were significantly suppressed, TEM show typical characteristics of apoptosis. The tubular VM can not form, with the down-regulating of VEGF mRNA expression, protein expression and secretion. Reversely, PMA promoted cell proliferation, invasion and migration, Morphological examination showed proliferation characteristics. The formation of tubular VM, accompanied with the up-regulating of VEGF mRNA expression, protein expression and secretion. Number of invaded cells, number of migrated cells, VEGF mRNA, VEGF protein expression, VEGF protein secretion for the control
group, DMS group, PMA group were as follows: the number of invaded cells were:112 ± 6.25;57 ± 8.00;142 ± 5.57 respectively. the number of migrated cells were: 69.33 ± 4.04;42 ± 4.16;111 ± 8.03 respectively; VEGF mRNA: 1 vs 0.74 ± 0.122 Thiamet G vs 1.22 ± 0.075; VEGF protein expression: 0.39 ± 0.05 vs 0.23 ± 0.02 vs 0.65 ± 0.06; VEGF protein secretion: 103 ± 8.96 vs 63.89 ± 8.44 vs 201.01 ± 17.93, the index of multiple comparisons between groups shows significant difference. Conclusion: Sphk1 promotes cell proliferation, invation and migration and suppresses cell apoptosis, induces the VM formation in human HT-29 colon cancer cell line possibly by up-regulating VEGF expression and secretion. Key Word(s): 1. Vasculogenic Mimicry; 2. Sphingosine kinase 1; 3. Human colon cell; 4.