A 5% addition of mushroom (Pleurotus ostreatus) and rice bran (Oryza sativa L.) flour was made to all the composite noodles, including FTM30, FTM40, and FTM50. The investigation assessed the levels of biochemicals, minerals, and amino acids within the noodles, alongside their sensory properties. This was done in relation to a control sample comprised of wheat flour. Experimentally, the carbohydrate (CHO) level in FTM50 noodles was markedly lower (p<0.005) than the carbohydrate (CHO) content in all developed noodles and the five commercial brands, A-1, A-2, A-3, A-4, and A-5. The FTM noodles contained notably higher quantities of protein, fiber, ash, calcium, and phosphorus when contrasted with the control and commercial noodles. The lysine percentage within the protein efficiency ratio (PER), essential amino acid index (EAAI), biological value (BV), and chemical score (CS) of FTM50 noodles was superior to that of commercially produced noodles. The FTM50 noodles displayed a zero bacterial count, and their sensory characteristics conformed to the established standards of acceptability. Enhancing the nutritional content of noodles through a greater diversity of varieties, utilizing FTM flours, is suggested by the outcomes.
For the development of flavor precursors, cocoa fermentation is an integral process. However, many small-scale cocoa farmers in Indonesia, due to the low yields and extended fermentation time, often choose to directly dry their cocoa beans, resulting in a reduction in the development of flavor precursors and ultimately, a less desirable cocoa flavor. Consequently, this investigation sought to augment the flavor precursors, specifically free amino acids and volatile compounds, present in unfermented cocoa beans through hydrolysis, employing bromelain as the catalyst. With bromelain concentrations of 35, 7, and 105 U/mL, unfermented cocoa beans were hydrolyzed for durations of 4, 6, and 8 hours, respectively. An investigation of enzyme activity, hydrolysis levels, free amino acids, reducing sugars, polyphenols, and volatile compounds was subsequently carried out, utilizing unfermented and fermented cocoa beans as control groups, with unfermented beans as a negative control and fermented beans as a positive control. While the hydrolysis attained a maximum of 4295% at 105 U/mL for 6 hours, this value did not display statistically significant differences from the hydrolysis measured at 35 U/mL for 8 hours. Unfermented cocoa beans exhibit a superior polyphenol content and an inferior reducing sugar content in relation to this sample. The concentration of free amino acids, particularly hydrophobic ones including phenylalanine, valine, leucine, alanine, and tyrosine, saw a rise, as did the presence of desirable volatile compounds, such as pyrazines. see more Accordingly, bromelain-mediated hydrolysis appears to have contributed to an increase in flavor precursor quantities and the nuanced tastes of the cocoa bean.
Epidemiological analyses have indicated a positive trend between increased high-fat food intake and the increased prevalence of diabetes. The potential for an increased diabetes risk could be associated with exposure to organophosphorus pesticides, like chlorpyrifos. The frequently identified organophosphorus pesticide chlorpyrifos, when paired with a high-fat diet, still presents an unclear impact on glucose metabolism. The study investigated the consequences of chlorpyrifos exposure on glucose metabolism in rats, differentiating between those fed a normal-fat diet and those fed a high-fat diet. The investigation's findings revealed a drop in liver glycogen and a concurrent surge in glucose in the chlorpyrifos-treated groups. The chlorpyrifos treatment group demonstrated a remarkable enhancement of ATP consumption in the context of a high-fat diet in the rats. see more Serum levels of insulin and glucagon were unaffected by the chlorpyrifos treatment, however. Substantially, the liver ALT and AST levels displayed more pronounced alterations in the high-fat chlorpyrifos-exposed group compared to the normal-fat chlorpyrifos-exposed group. The administration of chlorpyrifos resulted in an augmented level of liver malondialdehyde (MDA) and a diminished activity of glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD) enzymes. The high-fat chlorpyrifos-treated group exhibited more substantial changes in these biomarkers. The results show that a high-fat diet could exacerbate the detrimental effect of chlorpyrifos exposure on glucose metabolism, a consequence of antioxidant damage in the liver observed in all dietary groups.
Aflatoxin M1, a milk-borne toxin, is a product of the liver's biochemical conversion of aflatoxin B1 (AFB1) and presents a significant risk to human health when present in milk. see more Milk consumption's potential for AFM1 exposure necessitates a valuable health risk assessment. This Ethiopian research initiative, the first of its kind, focused on assessing the exposure and risk assessment of AFM1 in raw milk and cheese. An enzyme-linked immunosorbent assay (ELISA) was employed to ascertain the levels of AFM1. The milk products uniformly tested positive for AFM1. The risk assessment was established by means of the margin of exposure (MOE), estimated daily intake (EDI), hazard index (HI), and cancer risk. The mean daily exposure indices for raw milk and cheese consumption were 0.70 ng/kg bw/day and 0.16 ng/kg bw/day, respectively. The data demonstrate a trend where mean MOE values were, in nearly every case, lower than 10,000, which could indicate a potential health issue. A mean HI value of 350 was observed in raw milk consumers, contrasting with 079 for cheese consumers, implying adverse health implications for individuals consuming substantial amounts of raw milk. For milk and cheese consumers, the mean cancer risk was calculated as 129 per 100,000 individuals per year for milk and 29 per 100,000 individuals per year for cheese, which indicates a low incidence of cancer. Therefore, further examination of potential risks from AFM1 in children, who consume more milk than adults, is justified.
Plum pits, a promising source of dietary protein, are unfortunately lost during processing. Human nutrition could greatly benefit from the recovery of these underexploited proteins. A supercritical carbon dioxide (SC-CO2) treatment was applied to plum kernel protein isolate (PKPI) to broaden its industrial applicability. The dynamic rheology, microstructure, thermal characteristics, and techno-functional properties of PKPI were analyzed under varying SC-CO2 treatment temperatures ranging from 30 to 70°C. The dynamic viscoelastic properties of SC-CO2-treated PKPIs, as demonstrated by the results, exhibited a higher storage modulus, loss modulus, and a reduced tan delta value compared to native PKPI, suggesting enhanced strength and elasticity in the gels. The microstructural analysis indicated protein denaturation at elevated temperatures, yielding soluble aggregates and, consequently, a higher heat requirement for the thermal denaturation of SC-CO2-treated samples. SC-CO2-treated PKPIs exhibited a reduction of 2074% in crystallite size and a decrease of 305% in crystallinity. PKPIs subjected to a temperature of 60 degrees Celsius exhibited the most extensive dispersibility, a remarkable 115-fold increase compared to the unaltered PKPI sample. SC-CO2 treatment paves a novel way to improve the techno-functional traits of PKPIs, thereby widening its applications in both the food and non-food industries.
The pursuit of microorganism control within the food industry has significantly influenced research in food processing technologies. Ozone treatment for food preservation has gained significant interest thanks to its potent oxidative properties, which exhibit impressive antimicrobial effectiveness, leading to no residual contamination of foods. In this review of ozone technology, ozone's properties and oxidizing capacity are detailed, including an analysis of the intrinsic and extrinsic factors affecting its ability to inactivate microorganisms in both gaseous and liquid ozone environments. The mechanisms of ozone's action against foodborne bacteria, fungi, mold, and biofilms are further explored. A detailed analysis of current scientific literature is presented in this review, focusing on the influence of ozone on the growth control of microorganisms, the preservation of food appearance and sensory characteristics, the maintenance of nutrient content, the overall improvement in food quality, and the extension of shelf life in various food items, including vegetables, fruits, meats, and grains. The multifaceted influence of ozone, whether gaseous or liquid, in food processing has spurred its adoption in the food industry, responding to evolving consumer demand for nutritious and convenient meals, even though elevated ozone levels can negatively impact the physical and chemical properties of some food items. Employing ozone and other hurdle techniques, the future of food processing looks to be exceptionally promising. The review highlights a critical gap in understanding the optimal utilization of ozone treatment for food, focusing on crucial parameters like ozone concentration and humidity for surface and food decontamination.
Researchers in China assessed the presence of 15 Environmental Protection Agency-regulated polycyclic aromatic hydrocarbons (PAHs) in a sample set encompassing 139 vegetable oils and 48 frying oils. High-performance liquid chromatography-fluorescence detection (HPLC-FLD) was used to complete the analysis. The limit of detection varied from 0.02 to 0.03 g/kg, while the limit of quantitation ranged from 0.06 to 1.0 g/kg. Recovery rates averaged between 586% and 906%. Comparing the polycyclic aromatic hydrocarbon (PAH) content of various oils, peanut oil showcased the highest mean, 331 grams per kilogram, in contrast to olive oil's lowest mean of 0.39 grams per kilogram. A staggering 324% of vegetable oils in China were found to breach the European Union's established maximum levels. Vegetable oils exhibited a lower concentration of total PAHs compared to frying oils. The average daily intake of PAH15, measured in nanograms of BaPeq per kilogram of body weight, varied from 0.197 to 2.051.