Aqueous solubility values were derived by rearranging the dose number (Dn) equation ( Amidon et al., 1995) into Eq. (2), and employing the Dn values as reported by Benet et al. (2011), only for the compounds for which the authors reported the experimental aqueous solubility. The dose employed for the

estimation of the solubility as function of the Dn was 30 mg. The reason for selecting this dose was based on an exploratory study initially performed for buspirone, where administered the dose for the CR formulation was 30 mg ( Sakr and Andheria, 2001a and Sakr and Andheria, 2001b). The aforementioned procedure allowed us to evaluate the impact of SCH 900776 mouse solubility, regardless of the selected dose. equation(2) Solubility=Dose/250mlDn Human jejunal effective permeability was obtained from the report by Lennernas (2007).

Peff values were converted to apparent passive permeability in Caco-2 cell monolayers (Papp,Caco-2 (10−6 cm/s)) employing the relationship reported by Sun and co-workers (Eq. (3)) ( Darwich et al., 2010 and Sun et al., 2002). This conversion was performed to account for the passive component of the intestinal permeability described within Peff, whereas the active component was explicitly accounted by the simulations of the learn more P-gp-mediated efflux (described below). equation(3) Papp,Caco-2=10LogPeff+0.54410.7224 The use of the aforementioned correlation entails some limitations mainly due to the limited number of compounds on which it is based (n = 13), the observed mild correlation (r2 = 0.85), and the associated wide prediction intervals. Thus, a note of caution is recommended before its application. Nevertheless,

for the work performed herein, once the Papp,Caco-2 range was obtained using the aforementioned correlation, the Papp,Caco-2 values were converted back to Peff in the ADAM model, using the same equation. This was done in order to estimate the absorption rate constant (ka,i) in each of segments of the ADAM model ( Jamei et al., 2009c). Enzyme kinetic parameters, i.e., intrinsic metabolic clearance (CLint), Vmax and Km, for CYP3A4-mediated metabolism in human liver Modulators microsomes (HLM) were obtained from the review by Bu for 113 compounds ( Bu, 2006). Reported Vmax and Km values were employed directly as no Fossariinae correlation was observed between them. The CYP3A4-mediated intrinsic metabolic clearance was calculated from the ratio between the Vmax and Km, assuming linear conditions (Vmax/Km). Vmax and Km values were limited, when possible, to those that in combination generated CLint,CYP3A4 values within the CLint,CYP3A4 range reported by Bu (2006). Transporter kinetic parameters, i.e., Jmax and Km, for the P-gp-mediated efflux in Caco-2 cell monolayers were obtained from the work of Troutman and Thakker (2003) for 8 different P-gp substrates.