7 cells were co-cultured with ST2 cells pre-stimulated
with 1,25(OH)(2)D-3. Osteoclast-like cells were detected by staining with tartrate-resistant acid phosphatase (TRAP).
Results: HMW-HA decreased RANKL mRNA and protein expressions, whereas inhibition of hyaluronic acid (HA) synthesis by 4-MU enhanced RANKL expression. Blockage of HA-CD44 binding by CD44 mAb suppressed HMW-HA-mediated inhibition of RANKL. Pull-down assay findings also revealed that HMW-HA transiently activated RhoA in ST2 cells and pre-treatment with CD44 mAb inhibited the activation of RhoA protein mediated by HMW-HA. Moreover pre-treatment with Selleck Fer-1 Rho kinase pathway inhibitors also blocked the inhibition of RANKL by HMW-HA. Co-culture system results showed that HMW-HA down-regulated differentiation into
osteoclast-like cells by RAW 264.7 cells induced by 1,25(OH)(2)D-3-stimulated ST2 cells.
Conclusions: These results indicated that HA-CD44 interactions down-regulate RANKL expression and osteoclastogenesis via activation of the Rho kinase pathway. (C) 2013 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.”
“Sixteen known compounds isolated from the whole plants of Euphorbia tangutica, including phorbol-13-actate ALK inhibition (1) previously synthesised and obtained from a natural source for the first time, were evaluated in vitro against a panel of human cancer cell lines using the MTT method. R428 molecular weight Among them, ergosterol (6) exhibited significant cytotoxic activity against HL-60 cell line with an IC50 value of 3.3 mu M, and 3 beta,5 alpha-dihydroxy-15 beta-cinnamoyloxy-14-oxolathyra-6Z,12 E-diene (7) also displayed moderate activity.”
“Objective: This study aims to compare short term outcomes and oncological value of laparoscopy assisted (LADG) and open distal gastrectomy (ODG) in the treatment
of early gastric cancer.
Methods: Meta-analysis of 12 studies, including three randomized controlled trials, published between 2000 and 2007, comparing laparoscopy assisted and open distal gastrectomy in 951 patients with early gastric cancer, was done. Outcomes of interest were operative data, lymph node clearance, postoperative recovery complications.
Results: Overall morbidity rate was significantly less with LADG (10.5% versus 20.1%, P = 0.003, OR 0.52, CI 0.34-0.8). A mean of 4.61 less number of lymph nodes dissected than ODG (CI -5.96, -3.26 P < 0.001) when all studies are included. There was no difference between the two groups in number of lymph nodes dissected when less than D2 lymphadenectomy was done (2.44 nodes less in LADG group, CI -5.52, 0.63; P = 0.12). LADG patients had less operative blood loss (mean of 151 ml, P < 0.001), less time to walking, oral intake and flatus. LADG patients had less length of hospital stay (5.7 days, P < 0.001), postoperative fever and pain. ODG group showed significantly less operative time.