35, P = 0.005), time spent in periphery (F1,66 = 4.85, P = 0.03), and velocity (F1,66 = 4.93, P = 0.03), as well www.selleckchem.com/products/BAY-73-4506.html as an interaction between treatment and condition (respectively: F1,66 = 6.56, P = 0.01; F1,66 = 8.45, P = 0.004; F1,66 = 7.73, P = 0.007; and F1,66 = 4.02, P = 0.04. Post hoc analysis indicated that Obx induced an increase in all four parameters as compared with the C and ObxFO groups (P-values of 0.02). In this test, we analysed three parameters: immobility, swimming, and climbing (Fig. 3). Regarding immobility, there was no effect of condition (F1,66 = 2.41, P = 0.12), but there was a significant effect of treatment (F1,66 = 47.05, P = 0.0001) and an interaction between these two factors (F1,66 = 9.95,

P = 0.002). Post hoc analysis revealed that the Obx group had an increased immobility this website frequency as compared with the C and ObxFO groups (P = 0.01). FO supplementation reduced the frequency of this behavior as compared with the non-supplemented groups (Fig. 3A). Regarding swimming (Fig. 3B), there was no effect of Obx (F1,66 = 1.90, P = 0.17). A main effect of treatment (F1,66 = 56.97, P = 0.0001)

and an interaction between treatment and condition (F1,66 = 12.19, P = 0.001) were detected. Post hoc analysis revealed that Obx rats swam less than the other groups (P = 0.001), and that FO increased the frequency of this behavior as compared with the non-supplemented groups (P = 0.001). There were no effects of treatment or Obx on climbing behavior, and there was no interaction between factors (respectively: F1,66 = 3.49, P = 0.68; F1,66 = 0.17, P = 0.06; and F1,66 = 0.006, P = 0.94). Main effects of treatment (F1,66 = 16.27, P < 0.0001) and condition (F1,66 = 7.51, P = 0.007) and an interaction between these factors (F1,66 = 4.36, P = 0.04) were detected for the percentage of time spent in open arms and for the percentage of time spent in closed arms (respectively: F1,66 = 35.57, P = 0.0001; F1,66 = 21.52, P = 0.0002; and F1,66 = 14.78, P = 0.0002). Post hoc analysis revealed that the Obx group showed more anxiety-like behaviors than the C and ObxFO groups, spending less time in the open

arms (P = 0.001) and more in the closed arms (P = 0.0001) (Fig. 4). Analysis of exploration time (Fig. 5A) showed a main effect of condition (F1,136 = 16.99, P < 0.0001), but there was no isometheptene effect of treatment (F1,136 = 1.64, P = 0.20) and there was no interaction between the factors (F1,136 = 0.01, P = 0.91). Post hoc analysis revealed that the C (P = 0.001), FO (P = 0.02) and ObxFO (P = 0.02) groups spent more time exploring the object in the new than in the old position. The Obx group showed no differences in exploration between the two positions (P = 0.18). Regarding frequency of exploration (Fig. 5B), there was a main effect of condition (F1,136 = 7.37, P = 0.007) and an interaction between condition and treatment (F1,136 = 6.34, P = 0.01), but no effect of treatment (F1,136 = 0.026, P = 0.87).