, 2012). Detailed information on the sampling stations is given in Table 1. Ten cruises were conducted in the morning every BGB324 purchase 3–4 days from 28 April to 1 June, except from 4 to 14 May. Zooplankton collections were made by vertical hauls of a plankton net (mouth diameter:

0.5 m, mesh size: 505 μm) from 1 m above the bottom to the surface. The base of the net was weighted with a 15-kg hammer. The filtered water volume was determined by the rope length multiplied by the mouth size (unit: m3). After collection, the zooplankton was immediately preserved in 5% formaldehyde. Organisms were identified to species level under a stereomicroscope ( Chen and Zhang, 1965 and Zheng et al., 1984). Temperature and salinity were measured in situ using a YSI 6600 multi-parameter water quality monitor. For the determination of surface chlorophyll a (Chl a) concentration, a 200-mL water sample was gently passed through a SP600125 0.45 μm cellulose filter and extracted with acetone (90%v/v) for 24 h at 4°C in darkness. The surface layer Chl a concentration (unit: mg m− 3) was then determined with a Turner design 10 AU fluorometer before and after acidification ( Parsons et al. 1984). One-way ANOVA (least significant difference or LSD) was used to test for differences among

stations for physical and biological parameters from the DNPP outflow water area (S2), the aquaculture area (S6) and the external area of Dapeng Cove (S5). A species was defined as dominant when Y, the dominance indicator, was ≥ 0.02 ( Xu & Chen 1989). Y was calculated as follows: Y = (ni/N) fi, where i Rebamipide is the sample number, ni is the ith species abundance, fi is the frequency of occurrence of species i, and N is the total abundance of all zooplankton species. The hierarchical cluster and multidimensional scaling (MDS) analyses of similarity among the sampling stations were computed on the basis of the Bray-Curtis similarity index and

log10(x + 1)-transformed data from the dominant species ( Clarke & Gorley 2006). Pearson’s correlation analysis was used to examine possible relationships between sea surface temperature, salinity and Chl a with zooplankton abundance. The tests were deemed significant when P < 0.05. The surface water temperature of Dapeng Cove rose from 28 April to 1 June and then maintained a high level of nearly 30°C after 20 May. Salinity ranged from 28.78 to 32.19 owing to the frequent rains during the survey period (Figure 2a). The Chl a concentration fluctuated widely from 3.22 to 25.57 mg m− 3 with an irregular temporal distribution ( Figure 2b). There were marked increases in surface water temperature at the water outflow of the DNPP (S2) and in Chl a concentration at S4 and S6 ( Table 2). The regional distributions of salinity did not differ significantly among S2, S5 and S6 (P > 0.05); however, temperature at S2 was significantly higher than that at S5 and S6 (F = 8.581, P < 0.01).