1 mM D Asp, a significantly increased the synthesis of LH occurs. In fact, whereas the control sample contained an LH value of 250 8 mIU mg pro tein, in the sample incubated with D Asp, the LH concen tration Abiraterone rose to 480 11. 4 mIU mg, p 0. 001. The same effects occurred also when the concen tration of D Asp in the medium was 1. 0 mM. In this case the increased LH synthesis was enhanced 2. 32 fold. but the increase was not propor tionate to the concentration of D Asp. In this experiment we also measured the concentration of cAMP and cGMP in the medium in which the pituitary gland was incubated with D Asp and found that the con centration of cGMP was significantly increased. A concen tration of 0. 1 mM D Asp in 60 min of incubation induced a significant increase in the synthesis of cGMP it raised 2.

5 fold, from a value of 1. 0 0. 2 pmol mg tissue to 2. 5 0. 4 pmol mg tissue. D Asp at the concentration of 1. 0 mM in the medium induced a 3. 1 fold increase in the synthesis of cGMP. However, the increase was not proportional to D Asp concentration, Inhibitors,Modulators,Libraries thus indicating that the minimum concentration of 0. 1 mM is already suf ficient to stimulate the pituitary to synthesize cAMP. this is similar to the effects of other described molecules on the pituitary gland. the basal levels. In the testes D Asp was Effects of D aspartate on the synthesis of testosterone and cAMP in rat Leydig cells When Leydig cells obtained from rat testes were incubated with 0. 1 mM D Asp, there was a significant 2. 4 fold increase in the synthesis of testosterone.

From a basal value of 34 3 ng testosterone Inhibitors,Modulators,Libraries 106 in Leydig cells, after D Asp treatment, testosterone was raised to 82 3 ng 106 cells. When the con centration of D Asp was 1. 0 mM in the medium, the increase Inhibitors,Modulators,Libraries was 2. 94 fold compared with the control. Thus, as occurred with LH synthesis in the pituitary gland, in Leydig cells the concen tration of 0. 1 mM D Asp also induced testosterone syn thesis significantly. We looked for the action of D Asp on the second messenger and found that following treatment of Leydig cells with 0. 1 mM D Asp, cAMP was increased 3. 1 fold compared with the control. When D Asp was administered at a levels of 0. 1 mM in the medium, from a value of 20 3. 0 pmol 106 in Leydig cells in the control, cAMP rose to 62 8. 1 pmol 106. When D Asp was administered at a level of 1. 0 mM in the medium, a 5.

25 fold increase was observed. These results thus mirrored the previously reported data in which it was demonstrated that the stimulation of Leydig cells is accompanied Inhibitors,Modulators,Libraries by an increase in cAMP. Biosynthesis of D aspartate in rat tissues D aspartate racemase The presence of D Asp in rat tissues leads to the question of the origin of D Asp. In order to determine Inhibitors,Modulators,Libraries whether D Asp is biosynthesized in vivo by conversion of L Asp to D Asp, we incubated a sample homogenized with Vismodegib dosing L Asp and then measured the D Asp generated.