The STR and Latrophilin/CL 1 like GPS site were contained in mBAI3, as in mBAI1 and mBAI2. Therefore, mBAI3 was assumed to become a G protein coupled receptors in the head having STR and a GPS domain. Although the practical importance of this big difference is not known, mbai3 has 4 TSRs, as does mBAI2, while mBAI1 has 5 TSRs. The conserved domains observed in mBAI3 provide some indication of its likely function. Thrombospondin 1 and thrombospondin 2, two TSR containing proteins of the TSP family, get antiangiogenic activity. In comparison, thrombospondin 3, which lacks TSRs, has no inhibitory activity on human dermal microvascular endothelial cell Cabozantinib clinical trial growth, confirming that TSRs generate the antiangiogenic activity of TSP2 and TSP1. The TSR includes two subdomains that’ll independently influence the method of neovascularization, and synthetic peptides derived from the TSR have been found to have strong antiangiogenic activity in vivo and in assays of EC func-tion. The WSXW and CSXTCXXXXXXRXR motifs were present in 3 TSRs, although not the TSR, in every 3 mBAIs. Actually, mBAI1 has yet another TSR before 1st TSR, nonetheless it is not shown in Fig. 2B. The CSVTCG concept was identified within the first TSR of mBAI3. It has been noted that the CSXTCXXXXXXRXR, Organism WSXW, and CSVTCG motifs take part in cell binding. In the TSR of-the three mBAIs, a BBXB motif occurs instead of a WSXW motif. BBXB, found adjacent to the WSXW motif, can also be a cell binding motif. Previous studies showed the peptide sequence CSVTCG inside the TSR of TSP1 interacts with a receptor glycoprotein, CD36. The CSVTCG peptide mediates the in vitro and in vivo inhibitory effects of TSP1 on ECs. The very first TSR of BAI3 may be very important to antiangiogenic action as it includes a CSVTCG motif for CD36 binding. mBAI3 even offers CSFTCG and CSVTCS sequences, just like the CSVTCG concept. Properdin, which includes 6 TSRs, plays an essential role entirely complement activity. But, properdin missing the last TSR, which offers the sequence CPVTCG, is unable to support the alternative pathway C3 convertase. Using the NCBI preserved site search, we also found that the 4 TSR areas of mBAI3 align properly with spondins, serine proteinase inhibitor with TSRs, and disintegrin metalloproteinases Capecitabine 154361-50-9 with TSRs. The GPS domain includes about 50 aa residues, including 1 cleavage site and 4 cysteine residues, in every homologous GPCRs, the GPS domain is found in the extracellular part of the receptors immediately next to the first transmembrane segment. The GPS domain includes a putative proteolytic site that appears to be conserved in a number of homologous adhesion GPCRs, the cleavage sites for the extracellular part of the receptors are observed in the C terminal amino acid residues of the GPS, while this region is poorly conserved among GPCRs.